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A new spectrofluorimetric method was developed for the determination of the trace amount of heparin (Hep). Using doxycycline (DC)–europium ion (Eu 3+ ) as a fluorescent probe, in the buffer solution of pH=8.9, Hep can remarkably enhance the fluorescence intensity of the DC–Eu 3+ complex at λ=612nm and the enhanced fluorescence intensity of Eu 3+ ion is in proportion to the...
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