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MicroRNA (miRNA) transcription is still not well understood until now. To increase the miRNA abundance, we stimulated miRNA transcription with CuSO 4 and knocked down Drosha enzyme using dsRNA in Drosophila S2 cells. The full length transcripts of bantam, miR-276a and miR-277, the 5′-end of miR-8, the 3′-end of miR-2b and miR-10 were obtained. We also conducted a series of miRNA promoter analysis...
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