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The aim of this study was to develop and validate a new analytical method for the determination of 4‐hydroxy‐2‐nonenal (4‐HNE) in biological samples while applying microextraction by packed sorbent as a sample preparation method and HPLC with UV–vis detection. Various microextraction by packed sorbent (MEPS) sorbents like C2, C8, C18, M1 (80% C8 and 20% SCX) and silica were used to separate 4‐HNE...
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