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Addition of 5 mg abscisic acid l−1 after 12 days' growth of Taxus chinensis suspension culture gave the greatest paclitaxel accumulation at 11 mg l−1, which was almost 5 times that of the control culture. The highest paclitaxel production, 18 mg l−1, was obtained using 5 mg abscisic acid l−1 and 20 mg methyl jasmonate l−1.
Taxus chinensis suspension cells were cultured in the modified Gamborg's B5 medium. Addition of 50 mg chitosan l−1, 60 μM methyl jasmonate and 30 μM Ag+ resulted in the greatest paclitaxel production, at 25 mg l−1 in the cultures, being almost 40 times higher than that of the control culture, 10 times higher than that of the culture exposed to Ag+, 6 times higher than that of the culture elicited...
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