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A simple, rapid, and ultrasensitive fluorescence strategy for adenosine triphosphate (ATP) detection was developed by using a FAM (carboxyfluorescein) labeled DNA (FAM-DNA). In this strategy, highly fluorescent FAM-DNA was used as a probe, and nanoceria (CeO2 NPs) acted as an efficient quencher. FAM-DNA attached to the surface of nanoceria through the coordination between the phosphate group of DNA...
It is known that DNA molecules can be used to build a various of complicated geometrical DNA nanostructures with programmable sequence design, and these DNA nanomaterials show a promising application in biotechnology and biomedicine. However, the construction of large-sized three dimensional DNA-based nanomaterials still remains a challenge. In this work, we propose a new strategy that only employs...
A simple fluorescent probe HBI-GR based on the combination of the fluorophore (p-HBI) in green fluorescent protein (GFP) and Guanine riboside (GR) for HSA was successfully synthesized. HBI-GR showed an obvious fluorescence enhancement toward HSA without interference from other proteins, amino acids, anions and commonly existing metal ions. HBI-GR exhibited high sensitivity towards HSA with a good...
The detection of Hg2+ has attracted considerable attention because of the serious health and environmental problems caused by it. Herein, a novel ratiometric fluorescent chemsensor (GQDs–SR) based on the fluorescence resonance energy transfer (FRET) process for detecting of Hg2+ was designed and synthesized with rhodamine derivative covalently linked onto graphene quantum dots. In this sensor, the...
Ascorbic acid (AsA), also known as vitamin C, is a vital small-molecule antioxidant with multiple functions in vivo. It’s the major natural antioxidant found in plants and is also an essential component of human nutrition. AsA plays a key role in many diseases-related biological metabolism. Therefore, sensitive and selective detection of AsA is greatly important in pharmaceutical, clinical and food...
In this paper, a colorimetric and “turn-on” fluorescent sensor (Py–Si–Fe 3 O 4 @SiO 2 NPs) for Hg 2+ detection was designed with pyrene derivative covalently grafted onto the surface of magnetic core/shell Fe 3 O 4 @SiO 2 nanoparticles using the silanol hydrolysis approach. The Py–Si–Fe 3 O 4 @SiO 2 inorganic–organic hybrid...
In this paper, a simple and green strategy, based on the electrophoresis deposition technology, was reported to prepare Ag nanoparticles (NPs) modified TiO 2 nanotube arrays (NTs). The morphologies of AgNPs, TiO 2 NTs and AgNPs/TiO 2 NTs were characterized by transmission electron microscope (TEM) and scanning electron microscope (SEM). The results demonstrate that the surface...
CuO nanofibers (NFs), prepared by electrospinning and calcination technologies, have been applied for the fabrication of glucose sensors with high sensitivity and selectivity. Cu(NO 3 ) 2 and polyvinylpyrrolidone (PVP) composite nanofibers were initially electrospun on the surface of indium tin oxide (ITO) glass, and then the CuO NFs–ITO electrode was formed simply by removing PVP...
A novel rhodamine-based fluorescent chemosensor (RND) was synthesized that contains two independent fluorophores and acts as a very sensitive, selective and reversible off–on probe for Hg(II). Importantly, this newly developed sensing system exhibited different fluorescent responses toward Hg(II) and Mg(II) at 589nm and 523nm, respectively. RND also displayed detectable color change upon treatment...
A facile green biosynthesis method has been successfully developed to prepare gold nanoparticles (AuNPs) of various core sizes (25±7nm) using a natural biomaterial, eggshell membrane (ESM) at ambient conditions. In situ synthesis of AuNPs-immobilized ESM is conducted in a simple manner by immersing ESM in a pH 6.0 aqueous solution of HAuCl 4 without adding any reductant. The formation of AuNPs...
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