Search results for: Hee Gon Kim

Items from 1 to 11 out of 11 results

article

Graph neural network‐based virtual network function deployment optimization

Hee‐Gon Kim, Suhyun Park, Stanislav Lange, Doyoung Lee, more

International Journal of Network Management > 31 > 6 > n/a - n/a

Software‐defined networking (SDN) and network function virtualization (NFV) help reduce the operating expenditure (OPEX) and capital expenditure (CAPEX) as well as increase the network flexibility and agility. However, since the network is more dynamic and heterogeneous than before, operators have problems to cope with the increased complexity of managing virtual networks and machines. This complexity...

article

Degradation of Organic Compounds in Actual Wastewater by Electro-Fenton Process and Evaluation of Energy Consumption

Hee-Gon Kim, Yong-Jin Ko, Soonjae Lee, Seok Won Hong, more

Water, Air, & Soil Pollution > 2018 > 229 > 10 > 1-10

We investigated the Fe-regeneration-based cathodic electro-Fenton process using commercial electrodes, to achieve reducing the initial Fe dose and consequent sludge accumulation. The obtained results provided insights into the cathodic electro-Fenton process using two-electrode electrochemical system. The grade 2 Ti mesh electrode could effectively regenerate Fe(II) at 1.2 V, whose potential was lower...

article

Phosphorous recovery from sewage sludge using calcium silicate hydrates

Chang-Gu Lee, Pedro J.J. Alvarez, Hee-Gon Kim, Seongpil Jeong, more

Chemosphere > 2018 > 193 > C > 1087-1093

Phosphorous is an essential limiting nutrient for which there is no substitute. Its efficient recovery from sewage treatment plants is important to mitigate both dependence on limited reserves of exploitable phosphate rock and eutrophication of surface waters. Here, we evaluate the use of calcium silicate hydrates (CSH) to recover phosphorous eluted from sewage sludge. Phosphorous elution experiments...

article

Comparative analysis of two types of methanol dehydrogenase from Methylophaga aminisulfidivorans MP^T grown on methanol

Hee Gon Kim, Gui Hwan Han, Dockyu Kim, Jong‐Soon Choi, more

Journal of Basic Microbiology > 52 > 2 > 141 - 149

Two types of methanol dehydrogenase (MDH) were obtained from a novel marine methylotrophic bacterium, Methylophaga aminisulfidivorans MP^T, grown on methanol. Type I MDH consisted of two identical dimers of α (65.98 kDa) and β (7.58 kDa) subunits organized to form the α₂β₂tetramer. Type II MDH contained an additional MxaJ protein (27.86 kDa) and had more specific activity than type I MDH. The K_m values...

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Purification, crystallization and preliminary X‐ray crystallographic analysis of a methanol dehydrogenase from the marine bacterium Methylophaga aminisulfidivorans MP^T

Jin Myung Choi, Hee Gon Kim, Jeong‐Sun Kim, Hyung‐Seop Youn, more

Acta Crystallographica Section F > 67 > 4 > 513 - 516

Methylophaga aminisulfidivorans MP^T is a marine methylotrophic bacterium that utilizes C₁ compounds such as methanol as a carbon and energy source. The released electron from oxidation flows through a methanol‐oxidizing system (MOX) consisting of a series of electron‐transfer proteins encoded by the mox operon. One of the key enzymes in the pathway is methanol dehydrogenase (MDH), which contains the...

article

Voltammetric detection of trimethylamine using immobilized trimethylamine dehydrogenase on an electrodeposited goldnanoparticle electrode

Young-Bong Choi, Hee Gon Kim, Gui Hwan Han, Hyug-Han Kim, more

Biotechnology and Bioprocess Engineering > 2011 > 16 > 4 > 631-637

A voltammetric enzyme electrode was developed based on nicotinamide-independent trimethylamine dehydrogenase (TMADH, EC 1.5.99.7), which catalyses the oxidation of trimethylamine (TMA) to dimethylamine and formaldehyde. A quaternized osmium hydrogel polymer, poly(vinylimidazole-[Os(4,4′-dimethyl-2,2′-bipyridine)₂Cl]^+/2+) with ethylamine (PVI-Os-EA), was prepared as a potential redox mediator in an...

article

Optimization of lab scale methanol production by Methylosinus trichosporium OB3b

Hee Gon Kim, Gui Hwan Han, Si Wouk Kim

Biotechnology and Bioprocess Engineering > 2010 > 15 > 3 > 476-480

Methylosinus trichosporium OB3b is a methanotrophic bacterium containing particulate methane monooxygenase (MMO), which catalyzes the hydroxylation of methane to methanol. The methanol is further oxidized to formaldehyde by methanol dehydrogenase (MDH). We developed a novel compulsory circulation diffusion system for cell cultivation. A methane/air mixture (1:1, v/v) was prepared in a tightly sealed...

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Isolation and taxonomic characterization of a novel type I methanotrophic bacterium

Hee Gon Kim, Gui Hwan Han, Chi-Yong Eom, Si Wouk Kim

Journal of Microbiology > 2008 > 46 > 1 > 45-50

A methane-oxidizing bacterium was isolated from the effluent of manure and its molecular and biochemical properties were characterized. The isolate was aerobic, Gram-negative, and non-motile. The organism had a type I intracytoplasmic membrane structure and granular inclusion bodies. The outer cell wall surface (S-layers) was tightly packed with cup-shaped structures. Colonies were light yellow on...

article

Purification, characterization, and cloning of trimethylamine dehydrogenase fromMethylophaga sp. strain SK1

Hee Gon Kim, Yan Kim, Heon Man Lim, Hyun-Jae Shin, more

Biotechnology and Bioprocess Engineering > 2006 > 11 > 4 > 337-343

Trimethylamine dehydrogenase (TMADH, EC 1.5.99.7), an iron-sulfur flavoprotein that catalyzes the oxidative demethylation of trimethylamine to form dimethylamine and formaldehyde, was purified fromMethylophaga sp. strain SK1. The active TMADH was purified 12.3-fold through three purification steps. The optimal pH and temperature for enzyme activity was determined to be 8.5 and 55°C, respectively....

article

Purification and characterization of a methanol dehydrogenase derived fromMethylomicrobium sp. HG-1 cultivated using a compulsory circulation diffusion system

Hee Gon Kim, Si Wouk Kim

Biotechnology and Bioprocess Engineering > 2006 > 11 > 2 > 134-139

Methanotrophs are microorganisms that possess the unique ability to utilize methane as their sole source of carbon and energy. A novel culture system, known as the compulsory circulation diffusion system, was developed for rapid growth of methanotrophic bacteria. Methanol dehydrogenase (MDH, EC 1.1.99.8) fromMethylomicrobium sp. HG-1, which belongs to the type 1 group of methanotrophic bacteria, can...

article

Optimization of methanol biosynthesis from methane using Methylosinus trichosporium OB3b

Sang Gui Lee, Jae Hwan Goo, Hee Gon Kim, Jeong-Il Oh, more

Biotechnology Letters > 2004 > 26 > 11 > 947-950

Methylosinus trichosporium OB3b oxidized methane to methanol in the presence of a high concentration of Cu²⁺. Further oxidation of methanol to formaldehyde was prevented by adding 200 mm NaCl which acted as a methanol dehydrogenase H inhibitor. The bacterium, 0.6 mg dry cell ml⁻¹, in methane/air (1:4, v/v) at 25 °C in 12.9 mm phosphate buffer (pH 7) containing 20 mm sodium formate and 200 mm NaCl...

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