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A rainfall event is an important factor that can greatly affect the water quality of a river, particularly in a tropical country where the seasonal variations of river water quality are mainly dominated by precipitation. Hence, the present study aimed to determine the changes in the physicochemical characteristics of the Batang Baram, a tropical river in Malaysia, due to a rainfall event. Two samplings...
In this study, we describe a method for discriminating pathogenic bacteria with a dye. First, we determined that among several colours tested, the sunset yellow pigment easily coloured Escherichia coli bacteria yellow. Next, we demonstrated that E. coli O157:H7, Shigella flexneri O301, Staphylococcus aureus and Bacillus subtilis could all be well marked by sunset yellow pigment. Finally, we performed...
The passenger domain gene of PmpD of swine Chlamydophila abortus CP 16 was amplified, subcloned into the pet-32a(+) vector, then sequenced, and expressed in Escherichia coli BL21(DE3). The target protein was identified by Western blotting. It was demonstrated by sequence analysis that the passenger domain gene of Pomp18 contained 1317 bp that encoded a protein of 439 amino acids. The homology of the...
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