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Blakeslea trispora is an ideal natural source of lycopene. Here, we genetically manipulated the bifunctional protein gene, carRA, encoding a phytoene synthase and lycopene cyclase, to enhance lycopene production. carR- and carRA-knockout and carA overexpressing strains were obtained after several rounds of selection. Following combinational fermentation for 6 d, the lycopene content in carR-knockout...
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