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Surface plasmon resonance (SPR) sensor provides a useful means to study the concentration change on the sensor chip. In this study, we developed a living cells adhesion behavior monitoring technology, using SPR biosensor to detect the refractive index change induced by the living C6 cells attachment. Combined with the cell images under microscope, the re-distribution of molecules between the cell-matrix...
In present investigation, we established an on-line microorganism monitoring method based on SPR biosensor. Combined with asymmetric PCR method, we choose 370nt nucleic acid fragment contain the conserved sequence of Staphylococcus aureus for SPR detect. The detect limit was 13.7 ng/ml. The detection result can be effectually improved when the secondary structure of PCR product was broken by degeneration...
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