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A novel off-on fluorescent probe, developed for imaging the hypoxic status of tumor cells by means of detecting nitroreductase (NTR), consisted of a naphthalimide fluorophore and 4-Nitrobenzyl chloroformate as exogenous hypoxia markers. The detection mechanism is based on the fact that nicotinamide adenine dinucleotide (NADH) is utilized by nitroreductase as an electron source to reduce nitroaromatics...
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