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A simple and reproducible method for the analysis of ampicillin in human serum was developed. Serum samples were extracted using solid-phase extraction disk cartridges containing a sorbent of styrene divinyl/benzene. Extracts were separated by reversed-phase C 18 high-performance liquid chromatography with UV detection at 220 nm. The mobile phase consisted of acetonitrile-10 mM NaH ...
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