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Taking advantage of the homogeneous and heterogeneous electrochemical biosensors, a simple, sensitive, and selective electrochemical biosensor is constructed by combining entropy-driven amplification (EDA) with DNA walker. This electrochemical biosensor realizes the biorecognition and EDA operation in homogeneous solution, which is beneficial to improve the recognition and amplification efficiency...
Electron spin resonance spectroscopy was applied to determining the antioxidant capacity of eight thiol-containing compounds, including reduced glutathione, N-acetyl-L-cycsteine, methimazole, captopril, and tiopronin with one thiol group, 1,4-dithioerythritol and 2,3-dimercapto-1-propanol with two thiol groups, as well as L-cystine with no free thiol group. Cu2+ ion gives an electron spin resonance...
Trypsin and its inhibitors are relevant to many physiological processes and diseases. In this study, a nanosensor capable of detecting trypsin and its inhibitors was designed based on the fluorescence resonance energy transfer (FRET) between upconversion nanoparticle (UCNP) and gold nanoparticle (AuNP). UCNP and AuNP were linked by a trypsin-sensitive peptide DDDDARC, forming the non-fluorescent UCNP-peptide-AuNP...
Simple, rapid, sensitive, and specific detection of cancer cells plays a pivotal role in the diagnosis and prognosis of cancer. A sandwich electrochemical biosensor was developed based on polyadenine (polydA)-aptamer modified gold electrode (GE) and polydA-aptamer functionalized gold nanoparticles/graphene oxide (AuNPs/GO) hybrid for the label-free and selective detection of breast cancer cells (MCF-7)...
A methodology for detection of the Cauliflower Mosaic Virus 35S(CaMV35S) promoter was developed to distinguish transgenic from non-transgenic soybean samples by using photoelectrochemical (PEC) biosensor. In this PEC biosensing system, the as-prepared gold nanoparticles-reduced graphene oxide acted as a nanocarrier to immobilize the thiol-functional probe (probe1), and the SiO2@CdTe quantum dots (QDs)...
Non-polar solvent dynamic microwave assisted extraction was firstly applied to the treatment of high-fat soybean samples. In the dispersive micro-solid-phase extraction (d-µ-SPE), the herbicides in the high-fat extract were directly adsorbed on metal-organic frameworks MIL-101(Cr). The effects of several experimental parameters, including extraction solvent, microwave absorption medium, microwave...
A “signal on” electrochemiluminescence (ECL) sensor for pentachlorophenol (PCP) detection was constructed based on the amplified ECL of luminol at a multiwalled carbon nanotubes@graphene oxide nanoribbons (MWCNTs@GONRs) modified electrode. Due to the good electrocatalytic activity of MWCNTs@GONRs toward luminol system, the oxidation peak current of luminol at the MWCNTs@GONRs modified electrode was...
This communication clearly highlights the importance and necessity for a comparison investigation between nitrogen-doped graphene (N–G) and graphene as a two-dimensional mat of metal nanoparticles (NPs). We presented Ag NPs as a model of metal NPs for fabricating Ag/N–G and Ag/graphene nanocomposites, respectively. Compared with Ag/graphene nanocomposites, the Ag/N–G nanocomposites could facilitate...
The electrochemistry and electrochemiluminescence (ECL) of novel three-dimensional nanostructured Ru(bpy) 32+ /Ni(OH) 2 microspheres were investigated for the first time. The negatively charged porous Ni(OH) 2 microspheres composed of Ni(OH) 2 nanowires were specifically designed to interact with Ru(bpy) 32+ . The large surface area and porous structure...
Graphene-CdS (G-CdS) nanocomposites were successfully prepared by CdS nanocrystals (CdS NCs) formed in situ on the surface of graphene sheets, using graphene oxide (GO) sheets with rich negatively charged carboxylic acid groups as starting materials. Compared with pure CdS NCs, the presence of the graphene doped in G-CdS nanocomposites could facilitate the electrochemical redox process of CdS NCs;...
In this article, the poly-calcon carboxylic acid (poly-CCA) film modified electrode was prepared by cyclic voltammetry (CV). Then, an electrochemical DNA biosensor was developed for detection of PML/RARA fusion gene in acute promyelocytic leukemia (APL) by using 18-mer single-stranded deoxyribonucleic acid as the capture probe. The capture probe was covalently attached through free amines on the DNA...
A novel solid-contact potentiometric sensor for ascorbic acid based on cobalt phthalocyanine nanoparticles (NanoCoPc) as ionophore was fabricated without any need of auxiliary materials (such as membrane matrix, plasticizer, and other additives). The electrode was prepared by simple drop-coating NanoCoPc colloid on the surface of a glassy carbon electrode. A smooth, bright and blue thin film was strongly...
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