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The isolation and characterization of a new metalloprotein containing Cu and Fe atoms is reported. The as-isolated Cu–Fe protein shows an UV–visible spectrum with absorption bands at 320nm, 409nm and 615nm. Molecular mass of the native protein along with denaturating electrophoresis and mass spectrometry data show that this protein is a multimer consisting of 14±1 subunits of 15254.3±7.6Da. Mössbauer...
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