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Oriental ginseng is an important medicinal plant that grows in 2 major forms or ecotypes, wild and domesticated. Each form differs conspicuously in root phenotype, but can be converted from one type to another by habitat. Here we show that the habitat-induced transformation of ginseng root phenotype was accompanied by alteration in cytosine methylation at a large number of 5′-CCGG-3′ sites detected...
The 7B chromosome of common wheat was microdissected from pollen mother cells of the 78 monosomic line of common wheat cv. Chinese Spring (CS). After proteinase K and DNA topoisomerase I treatments, the isolated chromosomes were subjected to 1–3 rounds of DOP-PCR amplification, which produced continuous DNA fragments ranging from 150 to 700 bp. Genomic Southern hybridization confirmed that the PCR...
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