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Bioconversion of (R,S)-mandelonitrile (R,S-MN) to prepare (R)-(−)-mandelic acid (R-MA) with nitrilase is an attractive method in industrial application. However, during this bioconversion by whole cells of Alcaligenes faecalis CCTCC M 208168, R-MA was found to inhibit its own production. To improve R-MA productivity, a new biocatalytic process of in situ product removal (ISPR) has been developed utilizing...
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