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At pH 4.8 and 50 °C, semi-continuous countercurrent saccharification was performed using α-cellulose. Every second day, solids and liquids were transferred countercurrently and liquid samples were taken to determine sugar concentration in each stage. Train 1 (5 mg protein/g biomass, 8 stages) achieved 87.8% glucose conversion and 102 g/L total sugar. Train 2 (2 mg/g, 8 stages) achieved 56.1% glucose...
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