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The effect of nonhydrolyzable guanine nucleotides on mammalian acetyl CoA carboxylase (ACC) activity was examined. Using porous rat adipocytes and crude fat cell homogenates to study metabolic pathway flux, GMPPNP and/or GTPγS inhibited [ 14 C] fatty acid formation by up to 95% when either [6- 14 C]glucose-6-phosphate or [1- 14 C]acetyl CoA was used as substrate...
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