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The response surface methodology was employed to study the extraction of acidic polysaccharides from the stem of Ephedra sinica Stapf. The quantitative effects of extraction temperature, time, number and ratio of water to raw material on yield of ephedra acidic polysaccharides were investigated with Box–Behnken design. The experimental data were fitted to three second-order polynomial equations using...
Three polysaccharide fractions A, B and C (PA, PB and PC) were acquired from the stems of Ephedra sinica Stapf by sequential extraction with cold water, hot water, and 1.0M NaOH, respectively. Immunosuppressive activities of PA, PB and PC were investigated by carbon clearance test, delayed-type hypersensitivity reaction and humoral immune response in vivo. Then PB was further isolated to afford four...
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