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This study aimed at the efficacy of sequential treatment of bone marrow‐derived mesenchymal stem cell secretion for busulfan‐treated azoospermia in mice. The conditioned media (CM) was obtained from bone marrow mesenchymal stem cells (MSCs) or 293 cells. Chemically induced azoospermia mice received 200 μl MSC‐CM or 293‐CM twice a week intravenously for three consecutive weeks. The histological assessment...
The present study was designed to investigate the therapeutic effect of bone marrow MSC‐derived factors on gonadotropic toxicity induced by busulfan in vivo. The conditioned media (CM) was obtained from MSCs in serum‐free incubation for 48 hr and concentrated ~25‐fold by ultrafiltration. The CM of HEK 293 cells was treated as control (293‐CM). MSC‐CM was injected into busulfan mice via caudal veins...
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