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In vitro activation of purified rat microsomal glutathione transferase 1 (MGST1) by S-nitrosylation has been recently reported. This study was designated to explore its in vivo relevance. Unexpectedly, we failed to detect S-nitrosylated MGST1 in rat liver microsomes treated with S-nitrosoglutathione (GSNO); neither did we observe MGST1 S-nitrosylation in endotoxin challenged rats. However, by using...
Chlorambucil (4-[p-[bis [2-chloroethyl] amino] phenyl]-butanoic acid; CMB) is a bifunctional alkylating agent that exhibits acquired drug resistance upon repeated dosing in human. This compound reacts with glutathione (γ-glutamylcysteinylglycine, GSH) both non-enzymatically and enzymatically in the presence of glutathione S-transferases (GSTs) to produce several GSH conjugates. The formation of GSH...
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