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Strain engineering for industrial production requires the improvement of tolerance to multiple unfavorable conditions. Here, we report global regulator libraries based on the CRISPR‐enabled trackable genome engineering to engineer tolerance against multiple inhibitors in Escherichia coli. Deep mutagenesis libraries were rationally designed, and constructed to target 34,340 mutations across 23 global...
Weeping is an important hydraulic parameter for the design and optimization of perforated plates because it determines the lower limit of the tray operation (vapor load). Therefore, an accurate prediction of the weep rate is very important. First, according to our theoretical analysis of the weeping phenomena of large‐scale perforated trays, we proposed three weep modes on a perforated tray: (1) random...