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There is an increasing need to type all 11 HLA loci for solid organ and HSCT Tx to achieve better donor/recipient pairing. However, the amplification step in the existing NGS workflow is cumbersome and error-prone because it requires multiple locus-specific PCR set up for each sample. Multiplexing 11 loci in a single tube greatly simplifies the workflow. Our aims are to obtain healthy balanced coverage,...
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