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The pheromone response pathway of the yeast Saccharomyces cerevisiae is a well‐established model for the study of G proteins and mitogen‐activated protein kinase (MAPK) cascades. Our longstanding ability to combine sophisticated genetic approaches with established functional assays has provided a thorough understanding of signalling mechanisms and regulation. In this report, we compare new and established...
Ideal reporter genes for temporal transcription programmes have short half‐lives that restrict their detection to the window in which their transcripts are present and translated. In an effort to meet this criterion for reporters of transcription in individual living cells, we adapted the ubiquitin fusion strategy for programmable N‐end rule degradation to generate an N‐degron version of green fluorescent...
To gain insights on cellular mechanisms regulating actin polymerization, we used the Virtual Cell to model fluorescence recovery after photobleaching (FRAP) and chromophore‐assisted laser inactivation (CALI) experiments on EGFP‐capping protein (EGFP‐CP). Modeling the FRAP kinetics demonstrated that the in vivo rate for the dissociation of CP from actin filaments is much faster (∼0.1 s−1) than that...
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