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An indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and time-resolved fluoroimmunoassay (TR-FIA) based on an anti-N-butylquinoxaline-2-carboxamide (BQCA) monoclonal antibody were standardized and validated for quinoxaline-2-carboxylic acid (QCA) screening in animal tissues and its performance were compared to HPLC. The sensitivities obtained for edible tissue extracts were 1.62 and...
To quantitatively determine tylosin and tilmicosin in edible animal tissues, a time-resolved fluoroimmunoassay (TRFIA) has been developed and validated. For this purpose, desmycosin-O-carboxymethoxylamine-BSA was fixed onto microtiter plates, standards and samples were loaded and, finally, diluted europium-labeled anti-tylosin antibodies were added. Results show that the limit of detection for tylosin...
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