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CGH is a technique used to identify unbalanced chromosomal aberrations in solid tumors. It provides a global overview of chromosomal gains and losses. Two variants are used in research and diagnostics, chromosomal CGH and array CGH. The major difference is within the detection system. In chromosomal CGH preparations of normal test chromosomes serve as the reference, on which tumor and normal DNA is...
Laser microdissection is a method to select cells and small tissue fragments for molecular pathologic investigations, both in research and in diagnostics. Tissue sections are prepared; for better orientation, nuclei can be stained slightly by hematoxyline. Tumor cells can thus be separated from desmoplastic stroma; different groups of tumor cells can be investigated separately, and can be compared...
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