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A labeling scheme is introduced that facilitates the measurement of accurate 13Cβ chemical shifts of invisible, excited states of proteins by relaxation dispersion NMR spectroscopy. The approach makes use of protein over-expression in a strain of E. coli in which the TCA cycle enzyme succinate dehydrogenase is knocked out, leading to the production of samples with high levels of 13C enrichment (30–40%)...
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