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Desmosome formation in MDCK cells was investigated using a Ca2+ shift. Following preliminary treatment with cycloheximide at 37°C, continued surface transport and subsequent endocytosis were minimized by incubating cells at 19°C to trap nascent glycoproteins within the Golgi body. Release into high Ca2+ medium (HCM) at 37°C resulted in junction formation as well as relocation of the Golgi body and...
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