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The reliability and completeness of 1 H NMR resonance assignment can be improved by the use of 13 C- 1 H HSQC correlation spectra on unlabelled protein samples using pulsed field gradients. This technique is illustrated on a 5.2 mM sample of the 79 residue Desulfovibrio vulgaris ferrocytochrome c 553 . Protons attached to the same carbon can be unambiguously...
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