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Coherent anti-Stokes Raman scattering (CARS) is a nonlinear Raman process in which pulsed light of at least two frequencies is mixed within a sample. By overlapping pump light at a frequency of omegap and Stokes light at omegas temporally and spatially, CARS light at a frequency of 2omegap-omegas is produced. When the frequency difference (omegap-omegas) can be tuned to specific vibrational resonances...
In this paper, we present the two-photon excitation (TPE) spectra of various fluorescent proteins measured using Fourier-transform nonlinear spectroscopy with a spectral resolution of 60 cm-1. By the use of an ultrabroadband pulse with a spectrum ranging from 700 to 1100 nm, the TPE spectra ranging from 350 to 550 nm are measured. We measured the absolute TPE spectrum of various fluorescent proteins...
We show a technique to measure the two-photon excitation spectrum of various fluorophores, based on Fourier transform nonlinear spectroscopy with the use of ultrabroadband laser (670-1100 nm).
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