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Anion-exchange chromatography using Q-sepharose was used to remove endotoxins. The optimized buffer conditions of pH, buffer concentration and NaCl concentration provided four log reductions in the endotoxin level (from 5x10 5 EU/ml to 10 EU/ml). In glycine-NaOH buffer (pH 8.0-10.5), the upper limit of the concentration of NaCl was 300 mM and that of glycine was 50 mM. For the Tris-HCl buffer...
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