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Non-mitogenic form of recombinant Human acidic fibroblast growth factor (nrhaFGF) retained the cardio and neuro-protective characters of the wild-type aFGF while decreased the potential side effects like mitogenesis activity. In this paper, nrhaFGF was successfully expressed in Escherichia coli and yielded about 5% of the total soluble protein in the 10L fermentation tank. the succeedent chromatography,...
Drug design is the approach of finding drugs by design using computational tools. When designing a new drug, the structure of the drug molecule can be modeled by classification of potential chemical compounds. Kernel Methods have been successfully used in classifying potential chemical compounds. Frequency of labeled paths has been proposed to map compounds into feature in order to classify the characteristics...
Non-mitogenic form of Human acidic fibroblast growth factor (nhaFGF) retained the cardio-and neuroprotective characters of the wild-type aFGF while decreased the potential side effects like mitogenesis activity. In this paper, the effect of acetate concentration on the growth and expression of recombinant nhaFGF system Escherichia coli BL21 (DE3) /pET3C-nhaFGF was investigated. Four fed- batch modes:...
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