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To reduce dependence on the expensive cofactor and effectively biotransform ethyl 4-chloro-3-oxobutanoate, l-glutamine and glycine were found to enhance the content of intracellular NADH and the reductase activity. Adding the mixture of 200mM of l-glutamine and 500mM of glycine to the reaction media, a 1.67-fold of reductase activity was increased over the control without the addition of the two compounds...
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