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Using the CNS of Lymnaea stagnalis a method is described for the rapid analysis of neurotransmitters and their metabolites using high performance liquid chromatography coupled with electrochemical detection. Tissue samples were homogenised in ice-cold 0.1M perchloric acid and centrifuged. Using a C 18 microbore column the mobile phase was maintained at a flow rate of 100μl/min and consisted...
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