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Synthesis of [3α- 3 H]7-dehydrocholesterol is described via protection of the 5,7-diene system in 7-dehydrocholesterol as the Diels-Alder adduct with 4-phenyl-1,2,4-triazoline-3,5-dione followed by oxidation of the hydroxyl group to give the 3-oxo adduct. Reduction of the keto adduct with [ 3 H]sodium borohydride produced the adduct of [3α- 3 H]7-dehydrocholesterol from which...
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