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A rise in intracellular free Ca2+ concentration ([Ca2+]i) is required to activate sperm of all organisms studied. Such elevation of [Ca2+]i can occur either by influx of extracellular Ca2+ or by release of Ca2+ from intracellular stores. We have examined these sources of Ca2+ in sperm from the sea squirt Ascidia ceratodes using mitochondrial translocation to evaluate activation and the Ca2+-sensitive...
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