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Lanthanide‐doped upconversion nanoparticles (UCNPs) can convert two or more lower‐energy near‐infrared photons to a single photon with higher energy, which makes them particularly suitable for constructing nanoprobes with large imaging depth and minimal interference of autofluorescence and light scattering from biosamples. Furthermore, they feature excellent photostability, sharp and narrow emissions,...
Ever since the first report of UC‐FRET in 2005, which introduced upconversion (UC) fluorescence into biosensing, imaging and medical applications, it has been growing more and more quickly during the past 15 years. In article number 1905084, Zhihong Liu and co‐workers review the efforts to improve the signal contrast of UC probes and sensors, which have gone beyond the FRET principle.
Herein we report that few‐atom silver nanoclusters (Ag NCs) can be effective energy acceptors for upconversion phosphors (UCPs). A luminescence resonance energy transfer (LRET) probe for biothiols was constructed by decorating UCPs with dithiol‐stabilized Ag NCs. Owing to the unique properties of ultrasmall NCs, properties which bridge the gap between those of small molecules and those of nanoparticles,...
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