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The practical use of cell‐based seasonal influenza vaccines is currently being considered in Japan. From the perspective of adventitious virus contamination, we assessed the suitability of NIID‐MDCK cells (NIID‐MDCK‐Cs) as a safe substrate for the isolation of influenza viruses from clinical specimens. We first established a sensitive multiplex real‐time PCR system to screen for 27 respiratory viruses...
We studied the evolution of the G gene in the new genotype ON1 of RSV detected from patients with acute respiratory infection in Japan. Phylogenetic analyses and the evolutionary timescale were obtained by the Bayesian MCMC method. We also analyzed p‐distance and positive selection sites. A new genotype ON1 emerged around 2001. The evolution rate was rapid (3.57 × 10−3 substitutions/site per year)...
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