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Objective To study the effect of the Twist gene on the migration of human hepatocellular carcinoma cells and the possible mechanisms involved. Methods RT-PCR was used to detect expression of the Twist gene in primary (Hep11) and recurrent (Hep12) cell lines from the same HCC patient. Hep11 cells were stably transfected with Twist-cDNA, and Hep12 cells were transiently transfected with Twist RNAi...
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