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In order to measure intracellular sodium concentrations in resting cells of Fibrobacter succinogenes S85 by 23 Na NMR spectrometry, two methodological aspects were studied. First, three different shift reagents (Dy(PPP i ) 7−2 , Tm(DOTP) 5− , and Dy(TTHA) 3− ) were tested for their ability to separate internal and external 23 Na NMR resonances....
The endoglucanase gene (endC) of Fibrobacter succinogenes BL2 encodes a protein of 620 amino acids (EGC) that shows similarity with family E1 cellulases, and particularly with EGB from F. succinogenes S85. Alignment of the amino acid sequence of family E1 cellulases revealed that EGC is composed of a N-terminal domain and a large catalytic domain of 453 residues containing an extension of 60 residues...
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