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Large DNA fragments were transferred to rice (Oryza sativa L.) by an Agrobacterium-mediated transformation protocol using the binary bacterial artificial chromosome (BIBAC) vector system. Calli derived from mature embryos of japonica rice cultivar H1493 were used as target tissues. LBA4404 with the pCH32 helper plasmid carrying virE and virG was found to be the most efficient strain for the transfer...
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