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Amino acids have been shown to affect the development of mammary gland (MG). However, it is unclear whether L‐arginine promotes the development of pubertal MG. Therefore, our study aims to explore the effect of L‐arginine on the development of MG in pubertal mice. To investigate its internal mechanism of action, we will use mouse mammary epithelial cells (mMECs) line. Whole‐mount staining showed that...