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A method for analyzing 26 types of steroids in egg matrix was developed. The method used liquid chromatography coupled with tandem mass spectrometry (LC–MS/MS) in electrospray ionization mode (ESI). The procedure involved extraction with acetonitrile and removal of phospholipids by zinc chloride (ZnCl 2 ) followed by SPE cleanup with a Plexa cartridge. The effect of ZnCl 2 on phospholipid...
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