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Two genes, MGFc40 and MGFc24, encoding different E peptides of mechano-growth factor (MGF), were obtained by a four-step PCR strategy and subcloned into pRSETC and pGEX-6p-1. Recombinant MGFc40 protein (4 mg l−1) was expressed and purified by affinity chromatography using His60 Ni Superflow. Recombinant MGFc24 protein was purified using a glutathione-Sepharose 4B column. After enzymatic cleavage...
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