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In this paper, the reproductive toxicity of male mice treated with Microcystis aeruginosa cell extracts containing microcystins was examined. In contrast to the control group, male mice exposed intraperitoneally to 3.33 or 6.67μg microcystins/kg body weight for 14 days had decreased mean body weight, and the mean absolute weight of the testes and epididymides was decreased. However, the mean relative...
Microcystins produced by freshwater cyanobacteria are potent hepatotoxins and can cause animal intoxications and human illnesses. In the present study, the effects of microcystins on the embryonic development of Kunming mice were determined using cell extracts of Microcystis aeruginosa from the Nanwan reservoir, China. Forty-eight pregnant mice were divided into four groups of 12 mice. Pregnant mice...
The subchronic oral toxicity of microcystin in common carp (Cyprinus carpio L.) was investigated in this study. The fish (mean body weight of 322±36g, n=10) were orally exposed to Microcystis by feeding with bloom scum at a dose of 50μg microcystins/kg body weight under laboratory conditions for 28 days. Growth assay results showed that microcystin could completely inhibit the growth of carp, but...
A full-length cDNA of neurotoxin (Hk2a) was isolated by RT-PCR of total RNA isolated from tentacles of Anthopleura sp. using degenerate oligonucleotide primers and 3',5'-RACE. The cDNA sequence of Hk2a encoded a polypeptide of 47 amino acids, which lacks a typical N-terminal signal sequences commonly found in proteins that are secreted via endoplasmic reticulum-Golgi pathway, indicating the possibility...
Three full-length phospholipase A 2 (PLA 2 ) cDNAs from sea snake Lapemis hardwickii venom were cloned and sequenced in our previous study. In order to investigate their biological functions, we established a fusion expression system for PLA 2 -9 in E. coli. The open reading frame encoding mature peptide of PLA 2 -9 was subcloned into the vector pTRX. The Trx-PLA ...
A full-length cDNA of cytolysin (Src I) was isolated from the tentacle of Sagartia rosea (a representative species in China) by reverse transcription polymerase chain reaction. The cDNA with an open reading frame of 648bp encodes a precursor protein of 216 amino acids, which contains a prepropeptide of 38 amino acids including a signal peptide of 19 amino acids and a propart of 19 amino acids. Lys-Pro...
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