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•This study investigates the expression of miRNA-206 in a sporadic model of ALS.•3.7 fold increase in miR-206 seen in gastrocnemius muscle of ALS-CSF injected rats.•Contrarily, ALS-CSF caused 2.05 fold down-regulation of miR-206 in biceps brachii.•De-regulation of miR-206 modulates the muscle responses in a differential manner.•Mir-206 could be explored for its potential role in disease diagnosis...
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