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Laminin-121, previously referred as to laminin-3, was expressed recombinantly in human embryonic kidney (HEK) 293 cells by triple transfection of full-length cDNAs encoding mouse laminin α1, β2 and γ1 chains. The recombinant laminin-121 was purified using Heparin-Sepharose followed by molecular sieve chromatography and shown to be correctly folded by electron microscopy and circular dichroism (CD)...
The interactions of cells with basement membranes are primarily mediated via the engagement of laminins by a group of integrin family proteins, including integrins α3β1, α6β1, α7β1 and α6β4. To explore the ligand-binding specificities of these laminin-binding integrins, we produced these integrins, including two α7β1 splice variants (α7X1β1 and α7X2β1), as soluble recombinant proteins and determined...
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