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In this paper we describe the cloning and optimization of a nitrilase for a regio- and stereo-specific synthesis of (3S)-3-cyano-5-methyl hexanoic acid (2) from isobutylsuccinonitrile (IBSN, 1). Ten representative plant and bacterial nitrilases have been cloned and their substrate specificity was studied using a fluorescent assay. The desired nitrilase AtNit1 from Arabidopsis thaliana was identified...
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