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REPRESSOR OF SILENCING1 (ROS1) encodes a DNA demethylase that actively removes DNA methylation. Mutation in ROS1 leads to transcriptional gene silencing of a T‐DNA locus that contains two genes, RD29A‐LUC and 35S‐NPTII, originally expressed in the C24 wild type. These units have different silencing regulation mechanisms: the former mechanism is dependent on small interfering RNA (siRNA)‐directed...
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