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Summary Jaborandi (Pilocarpus microphyllus) is the only known source of pilocarpine, and although this alkaloid is the only natural compoud used to treat glaucoma, very little is known about its metabolism. Calluses obtained from petioles of P. microphyllus leaves were partially immersed in MS (Murashige and Skoog) liquid medium containing different pH levels (4.8, 5.8, and 6.8), nutrient concentration...
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