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We report here a homogeneous fluorometric method for the assays of multiple proteinases based on one AuNPs–peptide–fluorophore conjugate as the substrate. The peptide portion of conjugates has cleavage sites for multiple proteinases. Once nanoprobes are fabricated by end-immobilizing fluorophore labeled peptide onto the surface of gold nanoparticles, proteinase is introduced and enzymatic cleavage...
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